B11: Rational tuning of plasma and liquid chemistry for biocatalysis

Principal Investigators: Tim Dirks, Judith Golda

Team

Dirks, Tim (Principal Investigator)

Golda, Judith (Principal Investigator)

Klopsch, Sabrina (PhD)

Schöne, Anna Lena (PhD)

Minke, Robin (PhD)

Schüttler, Steffen (PostDoc)

Description

The aim is to optimise plasma-assisted biocatalysis in order to improve cost efficiency and industrial applicability. Central to this is the evaluation of more cost-effective feed gases - oxygen, nitrogen and synthetic air - as alternatives to the helium-based system used in previous funding periods. Evaluating how these gases affect the formation of reactive species, in particular H2O2 and NO, using gas and liquid phase diagnostics under all conditions, allowing a direct comparison of their effects on enzyme activity, stability and availability of co-substrates.

Publications

• Tuning plasma chemistry by various excitation mechanisms for the H₂O₂ production of atmospheric pressure plasma jets
  S. Schüttler, N. Eichstaedt, J. Golda
  J. Phys. D: Appl. Phys. 58, 025203 (2025)
  Repository: https://rdpcidat.rub.de/node/1012
• Transportation behaviour of OH and H₂O₂ in plasma-treated water
  H.-F. Poggemann, S. Schüttler, A. L. Schöne, E. Jeß, L. Schücke, T. Jacob, A. R. Gibson, J. Golda, C. K. Jung
  J. Phys. D: Appl. Phys. 58, 135208 (2025)
  Repository: https://rdpcidat.rub.de/node/1090
• Influence of humidity on the three-dimensional OH distribution in the effluent of the COST-Jet
  Robin Labenski*, Sebastian Burhenn, Maike Kai, Pia Pottkämper, Marc Böke, Volker Schulz-von der Gathen and Judith Golda
  J. Phys. D: Appl. Phys. 58 235202 (2025)
  Repository: https://rdpcidat.rub.de/node/1157
• The CYP152-family P450 enzyme CypC of Bacillus subtilis converts non- natural substrates in plasma-driven biocatalysis
  Tim Dirks, Sabrina Klopsch, Davina Stoesser, Sophie Desdemona Trenkle, Abdulkadir Yayci, Steffen Schüttler, Judith Golda, Julia Elisabeth Bandow
  Applied Microbiology and Biotechnology (2025) 109:193
  
• The Atmospheric Pressure Capillary Plasma Jet Is Well-Suited to Supply H2O2 for Plasma-Driven Biocatalysis
  Tim Dirks, Davina Stoesser, Steffen Schüttler, Frank Hollmann, Judith Golda, Julia E. Bandow
  ChemistryOpen 2025, 14, e202500057
  
• Control of the chemistry of a humidified He RF atmospheric pressure plasma jet by pulse modulation
  Steffen Schüttler, Maike Kai, Emanuel Jeß, Sebastian Burhenn, Laura Chauvet and Judith Golda
  Plasma Sources Sci. Technol. 34 105020 (2025)
  
• Validation of in situ diagnostics for the detection of OH and H₂O₂ in liquids treated by a humid atmospheric pressure plasma jet
  S. Schüttler, L. Jolmes, E. Jeß, K. Tschulik, J. Golda
  Plasma Process. Polym. 21, e2300079 (2024)
  Repository: https://rdpcidat.rub.de/node/720
• Comparison of CO production and Escherichia coli inactivation by a kHz and a MHz plasma jet
  E. Mestre, I. Orel, D. Henze, L. Chauvet, S. Burhenn, S. Dozias, F. Brulé-Morabito, J. Golda, C. Douat
  Plasma Process Polym. 21, e2300182 (2024)
  
• Production and transport of plasma-generated hydrogen peroxide from gas to liquid
  S. Schüttler , A. L. Schöne , E. Jeß, A. R. Gibson, J. Golda
  Phys. Chem. Chem. Phys. 26, 8255-8272 (2024)
  Repository: https://rdpcidat.rub.de/node/807
• Nitrogen fixation and H₂O₂ production by an atmospheric pressure plasma jet operated in He - H₂O - N₂ - O₂ gas mixtures
  S. Schüttler, J. Kaufmann, J. Golda
  Plasma Process. Polym. 21, e2300233 (2024)
  Repository: https://rdpcidat.rub.de/node/822
• The cold atmospheric pressure plasma-generated species superoxide, singlet oxygen, and atomic oxygen activate the molecular chaperone Hsp33
  T. Dirks, M. Krewing, K. Vogel, J. E. Bandow
  J. R. Soc. Interface 20, 20230300 (2023)
  Repository: https://rdpcidat.rub.de/node/784
• Immobilization protects enzymes from plasma-mediated inactivation
  T. Dirks, A. Yayci, S. Klopsch, M. Krewing, W. Zhang, F. Hollmann, J. E. Bandow
  J. R. Soc. Interface 20, 20230299 (2023)
  Repository: https://rdpcidat.rub.de/node/763
• State enhanced actinometry in the COST microplasma jet
  D. Steuer, H. van Impel, A. R. Gibson, V. Schulz-von der Gathen, M. Böke, J. Golda
  Plasma Sources Sci. Technol. 31, 10LT01 (2022)
  Repository: https://rdpcidat.rub.de/node/563
• RF-driven atmospheric-pressure capillary plasma jet in a He/O₂ gas mixture: Multi-diagnostic approach to energy transport
  T. Winzer, D. Steuer, S. Schüttler, N. Blosczyk, J. Benedikt, J. Golda
  J. Appl. Phys. 132, 183301 (2022)
  
• Characterization of three novel DyP-type peroxidases from Streptomyces chartreusis NRRL 3882
  A. Yayci, N. Bachmann, T. Dirks, E. Hofmann, J.E. Bandow
  J. Appl. Microbiol. 133, 2417-2429 (2022)
  

Thesis

• Steffen Schüttler, PhD thesis - 04/2025
  Plasma chemistry of H₂O₂ generation in atmospheric pressure plasma jets: from gas to liquid phase
• Maike Kai, Master thesis - 04/2023
  Diagnostics of plasma chemistry in atmospheric pressure plasmas operated in humid feed gas
• Océane Michels, Bachelor thesis - 08/2022
  Plasma-driven biocatalysis with the unspecific peroxygenase from Collariella virescens
• Lara Boeddinghaus, Bachelor thesis - 03/2023
  Diagnostik der Anregungsdynamik in einem Atmosphärendruckplasmajet mittels phasenaufgelöster optischer Emissionsspektroskopie
• Anna Lena Schöne, PhD thesis - ongoing
  Simulation of plasma and liquid chemistry for applications in biocatalysis
• Jannis Kaufmann, Bachelor thesis - 09/2023
  Diagnostik von plasmaerzeugten Stickstoff- und Sauerstoffspezies in Flüssigkeiten
• Sabrina Klopsch, PhD thesis - ongoing
  Biocatalysis scale-up concepts
• Niklas Eichstaedt, Bachelor thesis - 04/2024
  Untersuchung des Einflusses der Anregungsfrequenz auf die Produktion von plasmaerzeugtem Wasserstoffperoxid
• Emanuel Jeß, Bachelor thesis - 04/2022
  Diagnostics of Plasma-Produced Hydrogen Peroxide in Liquids
• Sarah Thiel, Bachelor thesis - 07/2024
  Expansion of plasma-driven biocatalysis: Epoxidation of unsaturated fatty acids with the unspecific peroxygenase from Collariella virescens
• Lukas Jasper, Bachelor thesis - 02/2023
  Unspecific Peroxygenase from Daldinia caldariorum in Escherichia coli for plasma-driven biocatalysis
• Leonidas Eliakopoulos, Bachelor thesis - 10/2024
  Untersuchung der zeitlichen und räumlichen Anregungsdynamik in einem mit kHz-Pulsen betriebenen Atmosphärendruckplasmajet
• Talisha Mroß, Bachelor thesis - 07/2025
  Rational tuning of plasma and liquid chemistry for biocatalysis

Further reading