B11: Rational tuning of plasma and liquid chemistry for biocatalysis

Principal Investigators: J. Bandow, J. Golda, A. Gibson

Team

Bandow, Julia (Principal Investigator)

Golda, Judith (Principal Investigator)

Klopsch, Sabrina (PhD)

Schüttler, Steffen (PhD)

Schöne, Anna Lena (PhD)

Eichstaedt, Niklas (Master)

Description

Plasma-driven biocatalysis seeks to use plasma-produced reactive species, such as hydrogen peroxide, as reactants for the enzymatic conversion of substrates into valuable products. A key to achieving industrial use of this approach is to produce reactants in sufficient amounts and with high energy efficiency. The aim of this project is to develop a rational scheme for tuning the plasma-biocatalysis system by combining expertise and insight from experimental plasma and liquid diagnostics, numerical plasma and liquid chemistry simulations and biotechnology. In addition to characterising rates and mechanisms of hydrogen peroxide generation, the formation of unwanted plasma species that are toxic to enzymes will be monitored under different plasma operating conditions. On the basis of simulation and experiment, new strategies will be developed to increase the biocatalytic yield.

Publications

• Tuning plasma chemistry by various excitation mechanisms for the H₂O₂ production of atmospheric pressure plasma jets
  S. Schüttler, N. Eichstaedt, J. Golda
  J. Phys. D: Appl. Phys. 58, 025203 (2025)
  Repository: https://rdpcidat.rub.de/node/1012
• Transportation behaviour of OH and H₂O₂ in plasma-treated water
  H.-F. Poggemann, S. Schüttler, A. L. Schöne, E. Jeß, L. Schücke, T. Jacob, A. R. Gibson, J. Golda, C. K. Jung
  J. Phys. D: Appl. Phys. 58, 135208 (2025)
  
• Validation of in situ diagnostics for the detection of OH and H₂O₂ in liquids treated by a humid atmospheric pressure plasma jet
  S. Schüttler, L. Jolmes, E. Jeß, K. Tschulik, J. Golda
  Plasma Process. Polym. 21, e2300079 (2024)
  Repository: https://rdpcidat.rub.de/node/720
• Comparison of CO production and Escherichia coli inactivation by a kHz and a MHz plasma jet
  E. Mestre, I. Orel, D. Henze, L. Chauvet, S. Burhenn, S. Dozias, F. Brulé-Morabito, J. Golda, C. Douat
  Plasma Process Polym. 21, e2300182 (2024)
  
• Production and transport of plasma-generated hydrogen peroxide from gas to liquid
  S. Schüttler , A. L. Schöne , E. Jeß, A. R. Gibson, J. Golda
  Phys. Chem. Chem. Phys. 26, 8255-8272 (2024)
  Repository: https://rdpcidat.rub.de/node/807
• Nitrogen fixation and H₂O₂ production by an atmospheric pressure plasma jet operated in He - H₂O - N₂ - O₂ gas mixtures
  S. Schüttler, J. Kaufmann, J. Golda
  Plasma Process. Polym. 21, e2300233 (2024)
  Repository: https://rdpcidat.rub.de/node/822
• The cold atmospheric pressure plasma-generated species superoxide, singlet oxygen, and atomic oxygen activate the molecular chaperone Hsp33
  T. Dirks, M. Krewing, K. Vogel, J. E. Bandow
  J. R. Soc. Interface 20, 20230300 (2023)
  Repository: https://rdpcidat.rub.de/node/784
• Immobilization protects enzymes from plasma-mediated inactivation
  T. Dirks, A. Yayci, S. Klopsch, M. Krewing, W. Zhang, F. Hollmann, J. E. Bandow
  J. R. Soc. Interface 20, 20230299 (2023)
  Repository: https://rdpcidat.rub.de/node/763
• State enhanced actinometry in the COST microplasma jet
  D. Steuer, H. van Impel, A. R. Gibson, V. Schulz-von der Gathen, M. Böke, J. Golda
  Plasma Sources Sci. Technol. 31, 10LT01 (2022)
  Repository: https://rdpcidat.rub.de/node/563
• RF-driven atmospheric-pressure capillary plasma jet in a He/O₂ gas mixture: Multi-diagnostic approach to energy transport
  T. Winzer, D. Steuer, S. Schüttler, N. Blosczyk, J. Benedikt, J. Golda
  J. Appl. Phys. 132, 183301 (2022)
  
• Characterization of three novel DyP-type peroxidases from Streptomyces chartreusis NRRL 3882
  A. Yayci, N. Bachmann, T. Dirks, E. Hofmann, J.E. Bandow
  J. Appl. Microbiol. 133, 2417-2429 (2022)
  

Thesis

• Emanuel Jeß, Bachelor thesis - 03/2021
  Diagnostics of plasma-produced hydrogen peroxide in liquids
• Steffen Schüttler, PhD thesis - ongoing
  Plasma chemistry of H₂O₂ generation in atmospheric pressure plasma jets: from gas to liquid phase
• Maike Kai, Master thesis - 04/2023
  Diagnostics of plasma chemistry in atmospheric pressure plasmas operated in humid feed gas
• Océane Michels, Bachelor thesis - 08/2022
  Plasma-driven biocatalysis with the unspecific peroxygenase from Collariella virescens
• Lara Boeddinghaus, Bachelor thesis - 03/2023
  Diagnostik der Anregungsdynamik in einem Atmosphärendruckplasmajet mittels phasenaufgelöster optischer Emissionsspektroskopie
• Anna Lena Schöne, PhD thesis - ongoing
  Simulation of plasma and liquid chemistry for applications in biocatalysis
• Jannis Kaufmann, Bachelor thesis - 09/2023
  Diagnostik von plasmaerzeugten Stickstoff- und Sauerstoffspezies in Flüssigkeiten
• Sabrina Klopsch, PhD thesis - ongoing
  Biocatalysis scale-up concepts
• Niklas Eichstaedt, Bachelor thesis - 04/2024
  Untersuchung des Einflusses der Anregungsfrequenz auf die Produktion von plasmaerzeugtem Wasserstoffperoxid
• Emanuel Jeß, Bachelor thesis - 04/2022
  Diagnostics of Plasma-Produced Hydrogen Peroxide in Liquids
• Sarah Thiel, Bachelor thesis - 07/2024
  Expansion of plasma-driven biocatalysis: Epoxidation of unsaturated fatty acids with the unspecific peroxygenase from Collariella virescens
• Lukas Jasper, Bachelor thesis - 02/2023
  Unspecific Peroxygenase from Daldinia caldariorum in Escherichia coli for plasma-driven biocatalysis
• Leonidas Eliakopoulos, Bachelor thesis - 10/2024
  Untersuchung der zeitlichen und räumlichen Anregungsdynamik in einem mit kHz-Pulsen betriebenen Atmosphärendruckplasmajet

Further reading