B8: Non-thermal plasma-driven biocatalysis

Principal Investigators: Julia Bandow

Team

Bandow, Julia (Principal Investigator)

Poggemann, Hanna-Friederike (PhD)

Description

Based on simulations and experiments, the H2O2-dependent biocatalysts rCviUPO and CypC are developed to perform better in plasma-assisted biocatalysis, which is evaluated by enzyme lifetime and total turnover number. By extending plasma-assisted biocatalysis beyond the comparatively stable plasma-derived species H2O2, we will pave the way for the utilisation of short-lived plasma species.

Publications

• Transportation behaviour of OH and H₂O₂ in plasma-treated water
  H.-F. Poggemann, S. Schüttler, A. L. Schöne, E. Jeß, L. Schücke, T. Jacob, A. R. Gibson, J. Golda, C. K. Jung
  J. Phys. D: Appl. Phys. 58, 135208 (2025)
  Repository: https://rdpcidat.rub.de/node/1090
• The CYP152-family P450 enzyme CypC of Bacillus subtilis converts non- natural substrates in plasma-driven biocatalysis
  Tim Dirks, Sabrina Klopsch, Davina Stoesser, Sophie Desdemona Trenkle, Abdulkadir Yayci, Steffen Schüttler, Judith Golda, Julia Elisabeth Bandow
  Applied Microbiology and Biotechnology (2025) 109:193
  
• The cold atmospheric pressure plasma-generated species superoxide, singlet oxygen, and atomic oxygen activate the molecular chaperone Hsp33
  T. Dirks, M. Krewing, K. Vogel, J. E. Bandow
  J. R. Soc. Interface 20, 20230300 (2023)
  Repository: https://rdpcidat.rub.de/node/784
• Immobilization protects enzymes from plasma-mediated inactivation
  T. Dirks, A. Yayci, S. Klopsch, M. Krewing, W. Zhang, F. Hollmann, J. E. Bandow
  J. R. Soc. Interface 20, 20230299 (2023)
  Repository: https://rdpcidat.rub.de/node/763
• An increase in surface hydrophobicity mediates chaperone activity in N-chlorinated RidA
  M. Varatnitskaya, J. Fasel, A. Müller, N. Lupilov, Y. Shi, K. Fuchs, M. Krewing, C. K. Jung, T. Jacob, B. Sitek, J. E. Bandow, K. S. Carroll, E. Hofmann, L. I. Leichert
  Redox Biol. 53, 102332 (2022)
  
• Protection strategies for biocatalytic proteins under plasma treatment
  A. Yayci, T. Dirks, F. Kogelheide, M. Alcalde, F. Hollmann, P. Awakowicz, J. E. Bandow
  J. Phys. D: Appl. Phys. 54, 035204 (2021)
  
• Microscale atmospheric pressure plasma jet as a source for plasma-driven biocatalysis
  A. Yayci, T. Dirks, F. Kogelheide, M. Alcalde, F. Hollmann, P. Awakowicz, J. E. Bandow
  ChemCatChem 12, 5893-5897 (2020)
  
• A dielectric barrier discharge plasma degrades proteins to peptides by cleaving the peptide bond
  M. Krewing, B. Schubert, J. E. Bandow
  Plasma Chem Plasma Process 40, 685 (2020)
  
• Plasma-driven in situ production of hydrogen peroxide for biocatalysis
  A. Yayci, A. Baraibar, M. Krewing, E. Fernandez Fueyo, F. Hollmann, M. Alcalde, R. Kourist, J. Bandow
  ChemSusChem 13, 2072 (2020)
  
• Plasma-driven Biocatalysis
  J. Bandow, A. Yayci, M. Krewing, R. Kourist, A. Gomez Baraibar
  International patent WO 2020/007576 A1, 09.01.2020
  
• Dielectric barrier discharge plasma treatment affects stability, metal ion coordination, and enzyme activity of bacterial superoxide dismutases
  M. Krewing, C. K. Jung, E. Dobbelstein, B. Schubert, T. Jacob, J. E. Bandow
  Plasma Process. Polym. 17, e2000019 (2020)
  
• Plasma-sensitive Escherichia coli mutants reveal plasma resistance mechanisms
  M. Krewing, F. Jarzina, T. Dirks, B. Schubert, J. Benedikt, J.-W. Lackmann, J. E. Bandow
  J. R. Soc. Interface 16, 20180846 (2019)
  
• The molecular chaperone Hsp33 is activated by atmospheric-pressure plasma protecting proteins from aggregation
  M. Krewing, J. J. Stepanek, C. Cremers, J.-W. Lackmann, B. Schubert, A. Müller, P. Awakowicz, L. Leichert, U. Jakob, J. E. Bandow
  J. R. Soc. Interface 16, 20180966 (2019)
  

Thesis

• Yayci Abdulkadir, PhD thesis - 10/2020
  Plasma-driven biocatalysis
• Cindy Juric, Bachelor thesis - 08/2018
  Identification of peroxidases in environmental samples for plasma-driven biocatalysis
• Tristan Hoffmann, Bachelor thesis - 08/2018
  Plasma stability of the copper-zinc superoxide dismutase of Escherichia coli
• Nathalie Gibas, Master thesis - 09/2018
  Identification and characterisation of peroxidases for plasma-driven biocatalysis
• Sabrina Klopsch, Bachelor thesis - 07/2019
  Plasma-driven biocatalysis with the vanadium-dependent chloroperoxidase from Curvularia inaequalis
• Tim Dirks, Master thesis - 09/2019
  Untersuchung der plasma-induzierten Aktivierung von Hsp33 aus Escherichia coli
• Marco Krewing, PhD thesis - 07/2019
  Impact of low-temperature plasmas on microorganisms and biomolecules
• Tim Dirks, PhD thesis - 06/2023
  Plasma-driven biocatalysis and the investigation of plasma-protein interactions
• Freya Jockenhövel, Bachelor thesis - 09/2020
  The chaperedoxin CnoX and its plasma-induced activation
• Ivan Bobkov, Bachelor thesis - 10/2020
  Effects of pre-adaptation and mutagenesis on the susceptibility of Escherichia coli to cold atmospheric plasma
• Denise Rittmann, Bachelor thesis - 07/2022
  Plasma-driven biocatalysis of the unspecific peroxygenase of Agrocybe aegerita with “ReliZyme”-beads
• Lukas Jasper, Bachelor thesis - 02/2023
  Unspecific Peroxygenase from Daldinia caldariorum in Escherichia coli for plasma-driven biocatalysis
• Hanna-Friederike Poggemann, PhD thesis - 12/2025
  Modeling of enzyme chemistry in biocatalysis
• Sabrina Klopsch, Master thesis - 09/2021
  Plasma-driven biocatalysis with the cytochrome P450 enzyme CYP152BSβ from Bacillus subtilis and the use of decoy molecule
• Sophie Trenkle, Bachelor thesis - 07/2024
  Expansion of plasma-driven biocatalysis using the enzymes CypC from Bacillus subtilis and TxtE from Streptomyces scabies

Further reading

• The role of VUV radiation in the inactivation of bacteria with an atmospheric pressure plasma jet
  S. Schneider, J. W. Lackmann, D. Ellerweg, B. Denis, F. Narberhaus, J. Bandow, J. Benedikt
  Plasma Process. Polym. 9, 561-568 (2012)
• Inactivation of microbes and macromolecules by atmosphericpressure plasma jets
  J. W. Lackmann, J. E. Bandow
  Appl. Microbiol. Biotechnol. 98, 6205-13 (2014)