B8: Non-thermal plasma-driven biocatalysis

Principal Investigators: J. Bandow, Ch. Jung

Team

Bandow, Julia (Principal Investigator)

Dirks, Tim (PostDoc)

Jung, Christoph (Principal Investigator)

Poggemann, Hanna-Friederike (PhD)

Description

Plasma-driven biocatalysis is a novel concept in which plasma-mediated in situ-generation of reactant is used to drive highly selective enzymatic syntheses. The applicability of plasma-driven biocatalysis is currently limited to the utilization of hydrogenperoxide by a handful of enzymes. The project goals are to identify novel plasma-biocatalysts with biotechnological potential and to accommodate their operating window by supplying optimal reactant concentrations and to establish a proof-of-principle for the utilization of other plasma-generated species in biocatalytic processes.

Publications

• The cold atmospheric pressure plasma-generated species superoxide, singlet oxygen, and atomic oxygen activate the molecular chaperone Hsp33
  T. Dirks, M. Krewing, K. Vogel, J. E. Bandow
  Journal of the Royal Society Interface 20:20230300 (2023)
  Repository: https://rdpcidat.rub.de/node/784
• Immobilization protects enzymes from plasma-mediated inactivation
  T. Dirks, A. Yayci, S. Klopsch, M. Krewing, W. Zhang, F. Hollmann, J. E. Bandow
  Journal of the Royal Society Interface 20:20230299 (2023)
  Repository: https://rdpcidat.rub.de/node/763
• Protection strategies for biocatalytic proteins under plasma treatment
  A. Yayci, T. Dirks, F. Kogelheide, M. Alcalde, F. Hollmann, P. Awakowicz, J. E. Bandow
  J. Phys. D: Appl. Phys. 54, 035204 (2021)
  
• Microscale atmospheric pressure plasma jet as a source for plasma-driven biocatalysis
  A. Yayci, T. Dirks, F. Kogelheide, M. Alcalde, F. Hollmann, P. Awakowicz, J. E. Bandow
  ChemCatChem 12, 5893-5897 (2020)
  
• A dielectric barrier discharge plasma degrades proteins to peptides by cleaving the peptide bond.
  M. Krewing, B. Schubert, J. E. Bandow
  Plasma Chem Plasma Process 40, 685 (2020)
  
• Plasma-driven in situ production of hydrogen peroxide for biocatalysis
  A. Yayci, A. Baraibar, M. Krewing, E. Fernandez Fueyo, F. Hollmann, M. Alcalde, R. Kourist, J. Bandow
  ChemSusChem 13, 2072 (2020)
  
• Plasma-driven Biocatalysis
  J. Bandow, A. Yayci, M. Krewing, R. Kourist, A. Gomez Baraibar
  International patent WO 2020/007576 A1, 09.01.2020
  
• Dielectric barrier discharge plasma treatment affects stability, metal ion coordination, and enzyme activity of bacterial superoxide dismutases
  M. Krewing, C. K. Jung, E. Dobbelstein, B. Schubert, T. Jacob, J. E. Bandow
  Plasma Process. Polym. e2000019 (2020)
  
• Plasma-sensitive E. coli mutants reveal plasma resistance mechanisms.
  M. Krewing, F. Jarzina, T. Dirks, B. Schubert, J. Benedikt, J.-W. Lackmann, J. E. Bandow
  J. R. Soc. Interface16:20180846 (2019)
  
• The molecular chaperone Hsp33 is activated by atmospheric-pressure plasma protecting proteins from aggregation
  M. Krewing, J. J. Stepanek, C. Cremers, J.-W. Lackmann, B. Schubert, A. Müller, P. Awakowicz, L. Leichert, U. Jakob, J. E. Bandow
  J. R. Soc. Interface 16:20180966 (2019)
  

Thesis

• Yayci Abdulkadir, PhD thesis - 07/2020
  Plasma-driven biocatalysis
• Cindy Juric, Bachelor thesis - 06/2018
  Identification of peroxidases in environmental samples for plasma-driven biocatalysis
• Tristan Hoffmann, Bachelor thesis - 08/2018
  Plasma stability of the copper-zinc superoxide dismutase of Escherichia coli
• Nathalie Gibas, Master thesis - 09/2018
  Identification and characterisation of peroxidases for plasma-driven biocatalysis
• Sabrina Klopsch, Bachelor thesis - 09/2019
  Undisclosed title
• Tim Dirks, Master thesis - 09/2019
  Untersuchung der plasma-induzierten Aktivierung von Hsp33 aus Escherichia coli
• Marco Krewing, PhD thesis - 07/2019
  Impact of low-temperature plasmas on microorganisms and biomolecules
• Tim Dirks, PhD thesis - 06/2023
  Plasma-driven biocatalysis and the investigation of plasma-protein interactions
• Freya Jockenhövel, Bachelor thesis - 09/2020
  The chaperedoxin CnoX and its plasma-induced activation
• Ivan Bobkov, Bachelor thesis - 10/2020
  Effects of pre-adaptation and mutagenesis on the susceptibility of Escherichia coli to cold atmospheric plasma
• Tim Dirks, Bachelor thesis - 07/2017
  Investigation of the plasma-induced activation of Escherichia coli Hsp33
• Denise Rittmann, Bachelor thesis - 10/2022
  Undisclosed title
• Lukas Jasper, Bachelor thesis - 06/2023
  Bio Catalysis
• Hanna-Friederike Poggemann, PhD thesis - ongoing
  

Further reading

• The role of VUV radiation in the inactivation of bacteria with an atmospheric pressure plasma jet,
  S. Schneider, J.W. Lackmann, D. Ellerweg, B. Denis, F. Narberhaus, J. Bandow, and J. Benedikt,
  Plasma Process. Polym. 9, 561-568 (2012)
• Inactivation of microbes and macromolecules by atmosphericpressure plasma jets,
  J.W. Lackmann and J.E. Bandow,
  Appl Microbiol Biotechnol 98, 6205-13 (2014)