Emile Carbone visits the research group experimental physics V
On Oktober 25th, 2019, Dr. Emile Carbone from the Max-Planck institute for plasma physics in Garching, Germany, is going to visit the research group of Prof. Czarnetzki. Dr. Carbone is research group leader of the group Plasma for Gas Conversion and is invited to the Ruhr-Universität Bochum in the framework of the CRC 1316. Moreover, he is going to have a seminar talk at experimental physics V about "CO2 dissociation by microwave plasmas: prospects and challenges" at 10:15 in NB 5/158. Everyone who is interested is welcome to join!
Plathinium conference in Antibes
The Coordinated Research Center CRC 1316 was presented at the 1st Plathinium Conference (September 16th until September 20th) in Antibes. This conference is a newly combined meeting of the former French vacuum congress, the French assembly on magnetron sputtering and thin films as well as the French conference on ion beam techniques. Achim von Keudell gave a plenary lecture on atmospheric pressure plasma chemistry and contributed with a tutorial on fundamentals of plasmas to a workshop preceding the meeting. Achim von Keudell was also elected to become the chair of the 2nd Plathinium conference in 2021.
Project Meeting Kerkrade
Between July 8 and July 10, 2019, the annual retreat of the CRC 1316 took place in Kerkrade. Nearly 40 participants joined the event at the Abbey Hotel Rolduc during the three days.
At the first day, a special PhD activity took place, followed by two days intensive scientific discussions with all participants.
PLASMA RESEARCH, project B7
Lightning bolt underwater
A plasma tears through the water within a few nanoseconds. It may possibly regenerate catalytic surfaces at the push of a button.
Electrochemical cells help recycle CO2. However, the catalytic surfaces get worn down in the process. Researchers at the Collaborative Research Centre 1316 “Transient atmospheric plasmas: from plasmas to liquids to solids” at Ruhr-Universität Bochum (RUB) are exploring how they might be regenerated at the push of a button using extreme plasmas in water. In a first, they deployed optical spectroscopy and modelling to analyse such underwater plasmas in detail, which exist only for a few nanoseconds, and to theoretically describe the conditions during plasma ignition. They published their report in the journal Plasma Sources Science and Technology on 4 June 2019.
A plasma tears through the water within a few nanoseconds. Following plasma ignition, there is a high negative pressure difference at the tip of the electrode, which results in ruptures forming in the liquid. Plasma then spreads across those ruptures.
Video: Experimentalphysik II
Plasmas are ionised gases: they are formed when a gas is energised that then contains free electrons. In nature, plasmas occur inside stars or take the shape of polar lights on Earth. In engineering, plasmas are utilised for example to generate light in fluorescent lamps, or to manufacture new materials in the field of microelectronics. “Typically, plasmas are generated in the gas phase, for example in the air or in noble gases,” explains Katharina Grosse from the Institute for Experimental Physics II at RUB.
Ruptures in the water
In the current study, the researchers have generated plasmas directly in a liquid. To this end, they applied a high voltage to a submerged hairline electrode for the range of several billionth seconds. Following plasma ignition, there is a high negative pressure difference at the tip of the electrode, which results in ruptures forming in the liquid. Plasma then spreads across those ruptures. “Plasma can be compared with a lightning bolt – only in this case it happens underwater,” says Katharina Grosse.
Hotter than the sun
Using fast optical spectroscopy in combination with a fluid dynamics model, the research team identified the variations of power, pressure, and temperature in these plasmas. “In the process, we observed that the consumption inside these plasmas briefly amounts to up to 100 kilowatt. This corresponds with the connected load of several single-family homes,” points out Professor Achim von Keudell from the Institute for Experimental Physics II. In addition, pressures exceeding several thousand bars are generated – corresponding with or even exceeding the pressure at the deepest part of the Pacific Ocean. Finally, there are short bursts of temperatures of several thousand degrees, which roughly equal and even surpass the surface temperature of the sun.
Water is broken down into its components
Such extreme conditions last only for a very short time. “Studies to date had primarily focused on underwater plasmas in the microsecond range,” explains Katharina Grosse. “In that space of time, water molecules have the chance to compensate for the pressure of the plasma.” The extreme plasmas that have been the subject of the current study feature much faster processes. The water can’t compensate for the pressure and the molecules are broken down into their components. “The oxygen that is thus released plays a vital role for catalytic surfaces in electrochemical cells,” explains Katharina Grosse. “By re-oxidating such surfaces, it helps them regenerate and take up their full catalytic activity again. Moreover, reagents dissolved in water can also be activated, thus facilitating catalysis processes.”
By Meike Drießen, Translated by Donata Zuber
RECENT RESEARCH ACHIEVEMENT, project B8
How bacteria protect themselves from plasma treatment
Plasmas are applied in the treatment of wounds to combat pathogens that are resistant against antibiotics. But bacteria know how to defend themselves.
Considering the ever-growing percentage of bacteria that are resistant to antibiotics, interest in medical use of plasma is increasing. In collaboration with colleagues from Kiel, researchers at Ruhr-Universität Bochum (RUB) investigated if bacteria may become impervious to plasmas, too. They identified 87 genes of the bacterium Escherichia coli, which potentially protect against effective components of plasma. “These genes provide insights into the antibacterial mechanisms of plasmas,” says Marco Krewing. He is the lead author of two articles that were published in the Journal of the Royal Society Interface this year.
A cocktail of harmful components stresses pathogens
Plasmas are created from gas that is pumped with energy. Today, plasmas are already used against multi-resistant pathogens in clinical applications, for example to treat chronic wounds. “Plasmas provide a complex cocktail of components, many of which act as disinfectants in their own right,” explains Professor Julia Bandow, Head of the RUB research group Applied Microbiology. UV radiation, electric fields, atomic oxygen, superoxide, nitric oxides, ozone, and excited oxygen or nitrogen affect the pathogens simultaneously, generating considerable stress. Typically, the pathogens survive merely several seconds or minutes.
In order to find out if bacteria, may develop resistance against the effects of plasmas, like they do against antibiotics, the researchers analysed the entire genome of the model bacterium Escherichia coli, short E. coli, to identify existing protective mechanisms. “Resistance means that a genetic change causes organisms to be better adapted to certain environmental conditions. Such a trait can be passed on from one generation to the next,” explains Julia Bandow.
Mutants missing single genes
For their study, the researchers made use of so-called knockout strains of E. coli. These are bacteria that are missing one specific gene in their genome, which contains approximately 4,000 genes. The researchers exposed each mutant to the plasma and monitored if the cells kept proliferating following the exposure.
“We demonstrated that 87 of the knockout strains were more sensitive to plasma treatment than the wild type that has a complete genome,” says Marco Krewing. Subsequently, the researchers analysed the genes missing in these 87 strains and determined that most of those genes protected bacteria against the effects of hydrogen peroxide, superoxide, and/or nitric oxide. “This means that these plasma components are particularly effective against bacteria,” elaborates Julia Bandow. However, it also means that genetic changes that result in an increase in the number or activity of the respective gene products are more capable of protecting bacteria from the effects of plasma treatment.
Heat shock protein boosts plasma resistance
The research team, in collaboration with a group headed by Professor Ursula Jakob from the University of Michigan in Ann Arbor (USA), demonstrated that this is indeed the case: the heat shock protein Hsp33, encoded by the hslO gene, protects E. coli proteins from aggregation when exposed to oxidative stress. “During plasma treatment, this protein is activated and protects the other E. coliproteins – and consequently the bacterial cell,” Bandow points out. An increased volume of this protein alone results in a slightly increased plasma resistance. Considerably stronger plasma resistance can be expected when the levels of several protective proteins are increased simultaneously.